Translational_Unit
Part:BBa_K2149001:Design
Designed by: Aline Larissa Gonçalves Group: iGEM16_USP-EEL-Brazil (2016-10-13)
HPPD from Pseudomonas putida
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 82
Illegal PstI site found at 964 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 82
Illegal PstI site found at 964 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 82
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 82
Illegal PstI site found at 964 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 82
Illegal PstI site found at 964
Illegal NgoMIV site found at 381
Illegal NgoMIV site found at 502 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 865
Design Notes
The gene was synthesized with thirty nucleotides in its ends to do a Gibson Assembly overlap. Its design takes into consideration the RBS and the coding sequence.
Source
This part was taken from the genomic sequence from Pseudomonas putida (access number K00457) that is a gram-negative, rod-shaped, saprotrophic soil bacterium. This gene is involved in the carotenoids pathway and was courtesy as a part of an operon that produces tocotrienol by Dr. Albermann.
References
ALBERMANN, C. et al. Biosynthesis of the vitamin E compound delta-tocotrienol in tecombinant Escherichia coli cells. ChemBioChem, v. 9, n. 15, p. 2524–2533, 2008.